Format

Send to

Choose Destination
Gene. 1989 Dec 21;85(1):109-14.

High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product.

Author information

1
Department of Genetics, Boehringer Mannheim GmbH, Penzberg (F.R.G.).

Abstract

We have observed that proteins, such as human tissue-type plasminogen activator, pro-urokinase or gp41 of human immunodeficiency virus, which have a high content of rare codons in their respective genes, are not readily expressed in Escherichia coli. Furthermore induction of these heterologous genes leads to growth inhibition and plasmid instability. Supplementation with tRNA(AGA/AGG(Arg)) by cotransfection with the dnaY gene, which supplies this minor tRNA, resulted in high-level production with greatly improved cell viability and plasmid stability.

PMID:
2515992
DOI:
10.1016/0378-1119(89)90470-8
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center