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Biochem Biophys Res Commun. 2014 Sep 26;452(3):322-7. doi: 10.1016/j.bbrc.2014.08.039. Epub 2014 Aug 23.

Anabolic androgens affect the competitive interactions in cell migration and adhesion between normal mouse urothelial cells and urothelial carcinoma cells.

Author information

1
Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan.
2
Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan; Department of Urology, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung, Taiwan.
3
Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan; George Whipple Lab for Cancer Research, Departments of Pathology, Urology and Radiation Oncology, University of Rochester Medical Center, Rochester, NY, United States.
4
Sex Hormone Research Center, Departments of Urology and Medical Technology, Graduate Institute of Clinical Medical Science, China Medical University/Hospital, Taichung 404, Taiwan. Electronic address: chshyr@mail.cmu.edu.tw.

Abstract

The urothelium is constantly rebuilt by normal urothelial cells to regenerate damaged tissues caused by stimuli in urine. However, the urothelial carcinoma cells expand the territory by aberrant growth of tumor cells, which migrate and occupy the damaged tissues to spread outside and disrupt the normal cells and organized tissues and form a tumor. Therefore, the interaction between normal urothelial cells and urothelial carcinoma cells affect the initiation and progression of urothelial tumors if normal urothelial cells fail to migrate and adhere to the damages sites to regenerate the tissues. Here, comparing normal murine urothelial cells with murine urothelial carcinoma cells (MBT-2), we found that normal cells had less migration ability than carcinoma cells. And in our co-culture system we found that carcinoma cells had propensity migrating toward normal urothelial cells and carcinoma cells had more advantages to adhere than normal cells. To reverse this condition, we used anabolic androgen, dihyrotestosterone (DHT) to treat normal cells and found that DHT treatment increased the migration ability of normal urothelial cells toward carcinoma cells and the adhesion capacity in competition with carcinoma cells. This study provides the base of a novel therapeutic approach by using anabolic hormone-enforced normal urothelial cells to regenerate the damage urothelium and defend against the occupancy of carcinoma cells to thwart cancer development and recurrence.

KEYWORDS:

Adhesion; Anabolic; Androgen; Migration; Urothelial carcinoma cell; Urothelial cell

PMID:
25159849
DOI:
10.1016/j.bbrc.2014.08.039
[Indexed for MEDLINE]

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