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Regen Med. 2014;9(4):453-65. doi: 10.2217/rme.14.13.

Human embryonic stem cell derived-mesenchymal stem cells: an alternative mesenchymal stem cell source for regenerative medicine therapy.

Author information

1
San Jose State University, 1 Washington Square, San Jose, CA 95192, USA.

Abstract

AIM:

To enumerate and characterize mesenchymal stem cells (MSC) derived from human embryonic stem cells (hESC) for clinical application.

MATERIALS & METHODS:

hESC were differentiated into hESC-MSC and characterized by the expression of surface markers using flow cytometry. hESC-MSC were evaluated with respect to growth kinetics, colony-forming potential, as well as osteogenic and adipogenic differentiation capacity. Immunosuppressive effects were assessed using peripheral blood mononuclear cell (PBMC) proliferation and cytotoxicity assays.

RESULTS:

hESC-MSC showed similar morphology, and cell surface markers as adipose (AMSC) and bone marrow-derived MSC (BMSC). hESC-MSC exhibited a higher growth rate during early in vitro expansion and equivalent adipogenic and osteogenic differentiation and colony-forming potential as AMSC and BMSC. hESC-MSC demonstrated similar immunosuppressive effects as AMSC and BMSC.

CONCLUSION:

hESC-MSC were comparable to BMSC and AMSC and hence can be used as an alternative source of MSC for clinical applications.

KEYWORDS:

AMSC; BMSC; differentiation; growth kinetics; hESC-MSC; immunosuppression

PMID:
25159063
DOI:
10.2217/rme.14.13
[Indexed for MEDLINE]

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