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Regen Med. 2014;9(4):437-52. doi: 10.2217/rme.14.26.

Immunohistochemical toolkit for tracking and quantifying xenotransplanted human stem cells.

Author information

1
Department of Pathology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.

Abstract

AIM:

Biomarker-based tracking of human stem cells xenotransplanted into animal models is crucial for studying their fate in the field of cell therapy or tumor xenografting.

MATERIALS & METHODS:

Using immunohistochemistry and in situ hybridization, we analyzed the expression of three human-specific biomarkers: Ku80, human mitochondria (hMito) and Alu.

RESULTS:

We showed that Ku80, hMito and Alu biomarkers are broadly expressed in human tissues with no or low cross-reactivity toward rat, mouse or pig tissues. In vitro, we demonstrated that their expression is stable over time and does not change along the differentiation of human-derived induced pluripotent stem cells or human glial-restricted precursors. We tracked in vivo these cell populations after transplantation in rodent spinal cords using aforementioned biomarkers and human-specific antibodies detecting apoptotic, proliferating or neural-committed cells.

CONCLUSION:

This study assesses the human-species specificity of Ku80, hMito and Alu, and proposes useful biomarkers for characterizing human stem cells in xenotransplantation paradigms.

KEYWORDS:

cell therapy; human-specific biomarker; image quantification; stem cells; xenotransplantation

PMID:
25159062
PMCID:
PMC4161450
DOI:
10.2217/rme.14.26
[Indexed for MEDLINE]
Free PMC Article

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