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FEBS J. 2014 Nov;281(21):4852-65. doi: 10.1111/febs.13024. Epub 2014 Sep 30.

Regulation of the catalytic activity of the human phosphatase PTPN4 by its PDZ domain.

Author information

1
Département de Biologie Structurale et Chimie, Unité de Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur, Paris, France; Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France.

Abstract

The human protein tyrosine phosphatase non-receptor type 4 (PTPN4) prevents cells death. Targeting its PDZ domain abrogates this protection and triggers apoptosis. We demonstrate here that the PDZ domain inhibits the phosphatase activity of PTPN4. The mere binding of a PDZ ligand is sufficient to release the catalytic inhibition. We combined analytical ultracentrifugation, small angle X-ray scattering and NMR to understand how the PDZ domain controls PTPN4 activity. We show that the physiologically active PTPN4 two-domain, encompassing the PDZ and the phosphatase domains, adopts a predominant compact conformation in solution. The PDZ ligand binding restores the catalytic competence of PTPN4 disrupting the transient interdomain communication. This study strengthens the emerging notion that PDZ domains can act as regulators of enzyme activity and therefore are active players in the dynamic regulation of signaling pathways.

KEYWORDS:

PDZ domain; enzymology; intramolecular regulation; protein dynamics; protein tyrosine phosphatase

PMID:
25158884
DOI:
10.1111/febs.13024
[Indexed for MEDLINE]
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