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Dev Biol. 2014 Oct 15;394(2):197-205. doi: 10.1016/j.ydbio.2014.08.014. Epub 2014 Aug 23.

Local and global dynamics of the basement membrane during branching morphogenesis require protease activity and actomyosin contractility.

Author information

1
Cell Biology Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Building 30, Room 403, Bethesda, MD 20892, USA.
2
Cell Biology Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Building 30, Room 403, Bethesda, MD 20892, USA. Electronic address: kenneth.yamada@nih.gov.

Abstract

Many epithelial tissues expand rapidly during embryonic development while remaining surrounded by a basement membrane. Remodeling of the basement membrane is assumed to occur during branching morphogenesis to accommodate epithelial growth, but how such remodeling occurs is not yet clear. We report that the basement membrane is highly dynamic during branching of the salivary gland, exhibiting both local and global remodeling. At the tip of the epithelial end bud, the basement membrane becomes perforated by hundreds of well-defined microscopic holes at regions of rapid expansion. Locally, this results in a distensible, mesh-like basement membrane for controlled epithelial expansion while maintaining tissue integrity. Globally, the basement membrane translocates rearward as a whole, accumulating around the forming secondary ducts, helping to stabilize them during branching. Both local and global dynamics of the basement membrane require protease and myosin II activity. Our findings suggest that the basement membrane is rendered distensible by proteolytic degradation to allow it to be moved and remodeled by cells through actomyosin contractility to support branching morphogenesis.

KEYWORDS:

Basement membrane; Branching morphogenesis; Matrix dynamics; Myosin II; Proteases

PMID:
25158168
PMCID:
PMC4174355
DOI:
10.1016/j.ydbio.2014.08.014
[Indexed for MEDLINE]
Free PMC Article
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