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Environ Pollut. 2014 Nov;194:246-253. doi: 10.1016/j.envpol.2014.07.024. Epub 2014 Aug 25.

'Rare biosphere' bacteria as key phenanthrene degraders in coastal seawaters.

Author information

1
CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, F-66650 Banyuls/mer, France; Sorbonne Universités, UPMC Univ Paris 06, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, F-66650 Banyuls/mer, France.
2
CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, F-66650 Banyuls/mer, France; CNRS, UMR 8222, Laboratoire d'Ecogéochimie des Environments Benthiques (LECOB), Observatoire Océanologique, F-66650 Banyuls/mer, France.
3
Aix-Marseille University, Mediterranean Institute of Oceanography (M I O), 13288 Marseille, Cedex 9, France; CNRS-INSU/IRD UM 110, Université du Sud Toulon-Var, 83957 La Garde Cedex, France.
4
CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, F-66650 Banyuls/mer, France; Sorbonne Universités, UPMC Univ Paris 06, UMR 7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, F-66650 Banyuls/mer, France. Electronic address: ghiglione@obs-banyuls.fr.

Abstract

By coupling DNA-SIP and pyrosequencing approaches, we identified Cycloclasticus sp. as a keystone degrader of polycyclic aromatic hydrocarbons (PAH) despite being a member of the 'rare biosphere' in NW Mediterranean seawaters. We discovered novel PAH-degrading bacteria (Oceanibaculum sp., Sneathiella sp.) and we identified other groups already known to possess this function (Alteromonas sp., Paracoccus sp.). Together with Cycloclasticus sp., these groups contributed to potential in situ phenanthrene degradation at a rate >0.5 mg l(-1) day(-1), sufficient to account for a considerable part of PAH degradation. Further, we characterized the PAH-tolerant bacterial communities, which were much more diverse in the polluted site by comparison to unpolluted marine references. PAH-tolerant bacteria were also members of the rare biosphere, such as Glaciecola sp. Collectively, these data show the complex interactions between PAH-degraders and PAH-tolerant bacteria and provide new insights for the understanding of the functional ecology of marine bacteria in polluted waters.

KEYWORDS:

DNA-SIP pyrosequencing; Keystone species; PAH-degraders; PAH-tolerant bacteria; Rare biosphere

PMID:
25156140
DOI:
10.1016/j.envpol.2014.07.024
[Indexed for MEDLINE]

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