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Methods Mol Biol. 2014;1196:319-48. doi: 10.1007/978-1-4939-1242-1_20.

Hox protein interactions: screening and network building.

Author information

1
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-5 box L7.07.10, Louvain-la-Neuve, 1348, Belgium.

Abstract

Understanding the mode of action of Hox proteins requires the identification of molecular and cellular pathways they take part in. This includes to characterize the networks of protein-protein interactions involving Hox proteins. In this chapter we propose a strategy and methods to map Hox interaction networks, from yeast two-hybrid and high-throughput yeast two-hybrid interaction screening to bioinformatic analyses based on the software platform Cytoscape.

PMID:
25151173
DOI:
10.1007/978-1-4939-1242-1_20
[Indexed for MEDLINE]

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