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Methods Mol Biol. 2014;1196:307-18. doi: 10.1007/978-1-4939-1242-1_19.

Bimolecular fluorescence complementation (BiFC) in live Drosophila embryos.

Author information

1
Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon - CNRS UMR5242, 46 Allée d'Italie, 69364, Lyon Cedex 07, France.

Abstract

Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general.

PMID:
25151172
DOI:
10.1007/978-1-4939-1242-1_19
[Indexed for MEDLINE]

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