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Nat Chem Biol. 2014 Oct;10(10):810-2. doi: 10.1038/nchembio.1610. Epub 2014 Aug 24.

Archaeal Elp3 catalyzes tRNA wobble uridine modification at C5 via a radical mechanism.

Author information

1
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Abstract

Approximately 25% of cytoplasmic tRNAs in eukaryotic organisms have the wobble uridine (U34) modified at C5 through a process that, according to genetic studies, is carried out by the eukaryotic Elongator complex. Here we show that a single archaeal protein, the homolog of the third subunit of the eukaryotic Elongator complex (Elp3), is able to catalyze the same reaction. The mechanism of action by Elp3 described here represents unprecedented chemistry performed on acetyl-CoA.

PMID:
25151136
PMCID:
PMC4479141
DOI:
10.1038/nchembio.1610
[Indexed for MEDLINE]
Free PMC Article

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