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Microbes Infect. 2014 Sep;16(9):746-54. doi: 10.1016/j.micinf.2014.08.003. Epub 2014 Aug 20.

Antibiotic susceptibility of Estrella lausannensis, a potential emerging pathogen.

Author information

1
Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland.
2
Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland. Electronic address: gilbert.greub@chuv.ch.

Abstract

Estrella lausannensis is a new Chlamydia-related bacterium, belonging to the Criblamydiaceae family. As suggested by its species name, this bacterium harbors a peculiar star shape. E. lausannensis is able to infect a wide range of amoebal, fish and mammalian cell lines. Moreover, seroprevalence of 2.9% was reported in children and in women with tubal pathology, showing that humans are commonly exposed to this recently discovered strict intracellular bacteria considered as a potential pathogen. Antibiotic susceptibility was determined using two approaches: qPCR and cellular mortality assay. Antibiotics classically used against intracellular bacteria were tested, including β-lactams, fluoroquinolones, cyclines and macrolides. We showed that E. lausannensis is resistant to β-lactams and fluoroquinolones, and sensitive to cyclines. Interestingly, E. lausannensis is slightly resistant to azithromycin with a MIC of 2 μg/ml, which is 10 fold higher compared to Waddlia chondrophila and Parachlamydia acanthamoebae MIC's. A single A2059C mutation in 23S rRNA gene could be responsible for this unexpected resistance.

KEYWORDS:

Antibiotic susceptibility; Cell culture; Chlamydia-like organism; Chlamydiae

PMID:
25150688
DOI:
10.1016/j.micinf.2014.08.003
[Indexed for MEDLINE]

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