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PLoS One. 2014 Aug 22;9(8):e103873. doi: 10.1371/journal.pone.0103873. eCollection 2014.

Generation and characterisation of novel pancreatic adenocarcinoma xenograft models and corresponding primary cell lines.

Author information

1
Cancer Stem Cell Innovation Centre and Department of Tumour Biology, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
2
Department of Genetics, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway; Department of Pathology, Oslo University Hospital, Oslo, Norway.
3
Department of Genetics, Institute of Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway; Department for Environmental Health and Science, Telemark University College, Telemark, Norway.

Abstract

Pancreatic adenocarcinoma is one of the most lethal cancer types, currently lacking efficient treatment. The heterogeneous nature of these tumours are poorly represented by the classical pancreatic cell lines, which have been through strong clonal selection in vitro, and are often derived from metastases. Here, we describe the establishment of novel pancreatic adenocarcinoma models, xenografts and corresponding in vitro cell lines, from primary pancreatic tumours. The morphology, differentiation grade and gene expression pattern of the xenografts resemble the original tumours well. The cell lines were analysed for colony forming capacity, tumourigenicity and expression of known cancer cell surface markers and cancer stem-like characteristics. These primary cell models will be valuable tools for biological and preclinical studies for this devastating disease.

PMID:
25148029
PMCID:
PMC4141735
DOI:
10.1371/journal.pone.0103873
[Indexed for MEDLINE]
Free PMC Article

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