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J Biomed Opt. 2014 Aug;19(8):086019. doi: 10.1117/1.JBO.19.8.086019.

Noncontact imaging of burn depth and extent in a porcine model using spatial frequency domain imaging.

Author information

1
Modulated Imaging Inc., Beckman Laser Institute Photonic Incubator, 1002 Health Sciences Road, Irvine, California 92617, United States.
2
Vanderbilt University, Department of Plastic Surgery, Cell and Developmental Biology, Nashville, Tennessee 37232, United States.

Abstract

The standard of care for clinical assessment of burn severity and extent lacks a quantitative measurement. In this work, spatial frequency domain imaging (SFDI) was used to measure 48 thermal burns of graded severity (superficial partial, deep partial, and full thickness) in a porcine model. Functional (total hemoglobin and tissue oxygen saturation) and structural parameters (tissue scattering) derived from the SFDI measurements were monitored over 72 h for each burn type and compared to gold standard histological measurements of burn depth. Tissue oxygen saturation (stO₂) and total hemoglobin (ctHbT) differentiated superficial partial thickness burns from more severe burn types after 2 and 72 h, respectively (p < 0.01), but were unable to differentiate deep partial from full thickness wounds in the first 72 h. Tissue scattering parameters separated superficial burns from all burn types immediately after injury (p < 0.01), and separated all three burn types from each other after 24 h (p < 0.01). Tissue scattering parameters also showed a strong negative correlation to histological burn depth as measured by vimentin immunostain (r² > 0.89). These results show promise for the use of SFDI-derived tissue scattering as a correlation to burn depth and the potential to assess burn depth via a combination of SFDI functional and structural parameters.

PMID:
25147961
PMCID:
PMC4141219
DOI:
10.1117/1.JBO.19.8.086019
[Indexed for MEDLINE]
Free PMC Article

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