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Science. 2014 Aug 22;345(6199):937-40. doi: 10.1126/science.1255091.

Riboswitches. A riboswitch-containing sRNA controls gene expression by sequestration of a response regulator.

Author information

1
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, TX 77030, USA.
2
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
3
Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
4
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA. danielle.a.garsin@uth.tmc.edu wwinkler@umd.edu.
5
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, TX 77030, USA. danielle.a.garsin@uth.tmc.edu wwinkler@umd.edu.

Abstract

The ethanolamine utilization (eut) locus of Enterococcus faecalis, containing at least 19 genes distributed over four polycistronic messenger RNAs, appears to be regulated by a single adenosyl cobalamine (AdoCbl)-responsive riboswitch. We report that the AdoCbl-binding riboswitch is part of a small, trans-acting RNA, EutX, which additionally contains a dual-hairpin substrate for the RNA binding-response regulator, EutV. In the absence of AdoCbl, EutX uses this structure to sequester EutV. EutV is known to regulate the eut messenger RNAs by binding dual-hairpin structures that overlap terminators and thus prevent transcription termination. In the presence of AdoCbl, EutV cannot bind to EutX and, instead, causes transcriptional read through of multiple eut genes. This work introduces riboswitch-mediated control of protein sequestration as a posttranscriptional mechanism to coordinately regulate gene expression.

Comment in

PMID:
25146291
PMCID:
PMC4356242
DOI:
10.1126/science.1255091
[Indexed for MEDLINE]
Free PMC Article

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