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Nat Rev Mol Cell Biol. 2014 Sep;15(9):591-600. doi: 10.1038/nrm3860.

RNAi screening comes of age: improved techniques and complementary approaches.

Author information

1
1] Drosophila RNAi Screening Center, Harvard Medical School, Boston, Massachusetts MA 02115, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts MA 02115, USA.
2
ICCB-Longwood Screening Facility, Harvard Medical School, Boston, Massachusetts MA 02115, USA.
3
Department of Genetics, Harvard Medical School, Boston, Massachusetts MA 02115, USA.
4
1] Drosophila RNAi Screening Center, Harvard Medical School, Boston, Massachusetts MA 02115, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts MA 02115, USA. [3] Howard Hughes Medical Institute, Boston, Massachusetts MA 02115, USA.

Abstract

Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells and in vivo in model organisms. These screens have resulted in the identification of new cellular pathways and potential drug targets. Considerable progress has been made to improve the quality of RNAi screen data through the development of new experimental and bioinformatics approaches. The recent availability of genome-editing strategies, such as the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system, when combined with RNAi, could lead to further improvements in screen data quality and follow-up experiments, thus promoting our understanding of gene function and gene regulatory networks.

PMID:
25145850
PMCID:
PMC4204798
DOI:
10.1038/nrm3860
[Indexed for MEDLINE]
Free PMC Article
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