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Cancer Res. 2014 Oct 15;74(20):5772-81. doi: 10.1158/0008-5472.CAN-14-0018. Epub 2014 Aug 21.

AEG-1 promoter-mediated imaging of prostate cancer.

Author information

1
Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, Maryland.
2
Department of Molecular and Comparative Pathobiology, Johns Hopkins Medical Institutions, Baltimore, Maryland.
3
Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, Virginia.
4
Department of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, Virginia. VCU Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, Virginia. VCU Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia.
5
Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, Maryland. mpomper@jhmi.edu.

Abstract

We describe a new imaging method for detecting prostate cancer, whether localized or disseminated and metastatic to soft tissues and bone. The method relies on the use of imaging reporter genes under the control of the promoter of AEG-1 (MTDH), which is selectively active only in malignant cells. Through a systemic, nanoparticle-based delivery of the imaging construct, lesions can be identified through bioluminescence imaging and single-photon emission computed tomography in the PC3-ML murine model of prostate cancer at high sensitivity. This approach is applicable for the detection of prostate cancer metastases, including bone lesions for which there is no current reliable agent for noninvasive clinical imaging. Furthermore, the approach compares favorably with accepted and emerging clinical standards, including PET with [(18)F]fluorodeoxyglucose and [(18)F]sodium fluoride. Our results offer a preclinical proof of concept that rationalizes clinical evaluation in patients with advanced prostate cancer.

PMID:
25145668
PMCID:
PMC4234089
DOI:
10.1158/0008-5472.CAN-14-0018
[Indexed for MEDLINE]
Free PMC Article
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