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BMC Genomics. 2014 Aug 22;15:701. doi: 10.1186/1471-2164-15-701.

Gene silencing via DNA methylation in naturally occurring Tragopogon miscellus (Asteraceae) allopolyploids.

Author information

1
Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand. j.tate@massey.ac.nz.

Abstract

BACKGROUND:

Hybridization coupled with whole-genome duplication (allopolyploidy) leads to a variety of genetic and epigenetic modifications in the resultant merged genomes. In particular, gene loss and gene silencing are commonly observed post-polyploidization. Here, we investigated DNA methylation as a potential mechanism for gene silencing in Tragopogon miscellus (Asteraceae), a recent and recurrently formed allopolyploid. This species, which also exhibits extensive gene loss, was formed from the diploids T. dubius and T. pratensis.

RESULTS:

Comparative bisulfite sequencing revealed CG methylation of parental homeologs for three loci (S2, S18 and TDF-44) that were previously identified as silenced in T. miscellus individuals relative to the diploid progenitors. One other locus (S3) examined did not show methylation, indicating that other transcriptional and post-transcriptional mechanisms are likely responsible for silencing that homeologous locus.

CONCLUSIONS:

These results indicate that Tragopogon miscellus allopolyploids employ diverse mechanisms, including DNA methylation, to respond to the potential shock of genome merger and doubling.

PMID:
25145399
PMCID:
PMC4148530
DOI:
10.1186/1471-2164-15-701
[Indexed for MEDLINE]
Free PMC Article

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