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PLoS One. 2014 Aug 21;9(8):e105633. doi: 10.1371/journal.pone.0105633. eCollection 2014.

Systematic regional variations in Purkinje cell spiking patterns.

Author information

1
Department of Neuroscience & Physiology, New York University School of Medicine, New York, New York, United States of America.
2
School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom.
3
Department of Systems Neurophysiology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
4
The Bionics Institute, East Melbourne, Victoria, Australia.
5
Department of Systems Neurophysiology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan; Center for Brain Integration Research, Tokyo Medical and Dental University, Tokyo, Japan.

Abstract

In contrast to the uniform anatomy of the cerebellar cortex, molecular and physiological studies indicate that significant differences exist between cortical regions, suggesting that the spiking activity of Purkinje cells (PCs) in different regions could also show distinct characteristics. To investigate this possibility we obtained extracellular recordings from PCs in different zebrin bands in crus IIa and vermis lobules VIII and IX in anesthetized rats in order to compare PC firing characteristics between zebrin positive (Z+) and negative (Z-) bands. In addition, we analyzed recordings from PCs in the A2 and C1 zones of several lobules in the posterior lobe, which largely contain Z+ and Z- PCs, respectively. In both datasets significant differences in simple spike (SS) activity were observed between cortical regions. Specifically, Z- and C1 PCs had higher SS firing rates than Z+ and A2 PCs, respectively. The irregularity of SS firing (as assessed by measures of interspike interval distribution) was greater in Z+ bands in both absolute and relative terms. The results regarding systematic variations in complex spike (CS) activity were less consistent, suggesting that while real differences can exist, they may be sensitive to other factors than the cortical location of the PC. However, differences in the interactions between SSs and CSs, including the post-CS pause in SSs and post-pause modulation of SSs, were also consistently observed between bands. Similar, though less strong trends were observed in the zonal recordings. These systematic variations in spontaneous firing characteristics of PCs between zebrin bands in vivo, raises the possibility that fundamental differences in information encoding exist between cerebellar cortical regions.

PMID:
25144311
PMCID:
PMC4140808
DOI:
10.1371/journal.pone.0105633
[Indexed for MEDLINE]
Free PMC Article

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