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Peptides. 1989 Sep-Oct;10(5):903-11.

Corticotropin-releasing factor release from in vitro superfused and incubated rat hypothalamus. Effect of potassium, norepinephrine, and dopamine.

Author information

1
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U297, Faculté de Médecine Nord Bd. P. Dramard, Marseille, France.

Abstract

We have compared the release of CRF induced by potassium depolarization, noradrenaline or dopamine as monitored either during superfusion of mediobasal hypothalamus or during incubation of whole hypothalamus. The superfusion device was improved in order to prevent gas leakage and to keep constant pO2 and pCO2 in the superfusion chamber. Basal CRF secretion as well as KCl- and norepinephrine-induced CRF release were comparable in superfusion and incubation experiments. Pharmacological investigations suggest that the stimulatory effect of norepinephrine on CRF release is mediated mainly through alpha 1 and alpha 2 adrenergic receptors, and partially through beta receptors.

PMID:
2514417
DOI:
10.1016/0196-9781(89)90167-8
[Indexed for MEDLINE]

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