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J R Soc Interface. 2014 Oct 6;11(99). pii: 20140672. doi: 10.1098/rsif.2014.0672.

Mapping the stereotyped behaviour of freely moving fruit flies.

Author information

1
Joseph Henry Laboratories of Physics and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
2
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
3
Joseph Henry Laboratories of Physics and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA shaevitz@princeton.edu.

Abstract

A frequent assumption in behavioural science is that most of an animal's activities can be described in terms of a small set of stereotyped motifs. Here, we introduce a method for mapping an animal's actions, relying only upon the underlying structure of postural movement data to organize and classify behaviours. Applying this method to the ground-based behaviour of the fruit fly, Drosophila melanogaster, we find that flies perform stereotyped actions roughly 50% of the time, discovering over 100 distinguishable, stereotyped behavioural states. These include multiple modes of locomotion and grooming. We use the resulting measurements as the basis for identifying subtle sex-specific behavioural differences and revealing the low-dimensional nature of animal motions.

KEYWORDS:

Drosophila; behaviour; phase reconstruction; stereotypy; unsupervised learning

PMID:
25142523
PMCID:
PMC4233753
DOI:
10.1098/rsif.2014.0672
[Indexed for MEDLINE]
Free PMC Article

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