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EMBO Rep. 2014 Oct;15(10):1077-84. doi: 10.15252/embr.201438793. Epub 2014 Aug 20.

Flexibility in crosstalk between H2B ubiquitination and H3 methylation in vivo.

Author information

1
Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands.
2
Biomolecular Mass Spectrometry and Proteomics Group, The Netherlands Proteomics Centre Utrecht University, Utrecht, the Netherlands.
3
Instituto de Biología Funcional y Genómica CSIC/University of Salamanca, Salamanca, Spain.
4
Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, the Netherlands fred.v.leeuwen@nki.nl.

Erratum in

  • EMBO Rep. 2014 Nov;15(11):1220-1.

Abstract

Histone H2B ubiquitination is a dynamic modification that promotes methylation of histone H3K79 and H3K4. This crosstalk is important for the DNA damage response and has been implicated in cancer. Here, we show that in engineered yeast strains, ubiquitins tethered to every nucleosome promote H3K79 and H3K4 methylation from a proximal as well as a more distal site, but only if in a correct orientation. This plasticity indicates that the exact location of the attachment site, the native ubiquitin-lysine linkage and ubiquitination cycles are not critical for trans-histone crosstalk in vivo. The flexibility in crosstalk also indicates that other ubiquitination events may promote H3 methylation.

KEYWORDS:

Dot1; Set1; chromatin; crosstalk; histone ubiquitination

PMID:
25141862
PMCID:
PMC4253848
DOI:
10.15252/embr.201438793
[Indexed for MEDLINE]
Free PMC Article

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