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PLoS One. 2014 Aug 20;9(8):e105684. doi: 10.1371/journal.pone.0105684. eCollection 2014.

HLA-B27 and human β2-microglobulin affect the gut microbiota of transgenic rats.

Author information

1
Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States of America.
2
Division of Rheumatology, University of Washington, VA Medical Center, Seattle, Washington, United States of America.
3
Division of Rheumatology, Oregon Health & Science University, Portland, Oregon, United States of America.
4
Moorfield's Eye Institute of London, London, United Kingdom.
5
Department of Ophthalmology, University of Washington, Seattle, Washington, United States of America.
6
Department of Molecular Microbiology & Immunology, Oregon Health & Science University, Portland, Oregon, United States of America.
7
Department of Rheumatology, University of Texas Southwestern, Dallas, Texas, United States of America.
8
University of Colorado Boulder, Boulder, Colorado, United States of America.
9
Pediatric Translational Research Branch, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Baltimore, Maryland, United States of America.
10
University of Colorado Boulder, Boulder, Colorado, United States of America; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, Colorado, United States of America.
11
Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States of America; Division of Rheumatology, Oregon Health & Science University, Portland, Oregon, United States of America; Dever's Eye Institute, Portland, Oregon, United States of America.

Abstract

The HLA-B27 gene is a major risk factor for clinical diseases including ankylosing spondylitis, acute anterior uveitis, reactive arthritis, and psoriatic arthritis, but its mechanism of risk enhancement is not completely understood. The gut microbiome has recently been shown to influence several HLA-linked diseases. However, the role of HLA-B27 in shaping the gut microbiome has not been previously investigated. In this study, we characterize the differences in the gut microbiota mediated by the presence of the HLA-B27 gene. We identified differences in the cecal microbiota of Lewis rats transgenic for HLA-B27 and human β2-microglobulin (hβ2m), compared with wild-type Lewis rats, using biome representational in situ karyotyping (BRISK) and 16S rRNA gene sequencing. 16S sequencing revealed significant differences between transgenic animals and wild type animals by principal coordinates analysis. Further analysis of the data set revealed an increase in Prevotella spp. and a decrease in Rikenellaceae relative abundance in the transgenic animals compared to the wild type animals. By BRISK analysis, species-specific differences included an increase in Bacteroides vulgatus abundance in HLA-B27/hβ2m and hβ2m compared to wild type rats. The finding that HLA-B27 is associated with altered cecal microbiota has not been shown before and can potentially provide a better understanding of the clinical diseases associated with this gene.

PMID:
25140823
PMCID:
PMC4139385
DOI:
10.1371/journal.pone.0105684
[Indexed for MEDLINE]
Free PMC Article

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