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Rev Bras Cir Cardiovasc. 2014 Apr-Jun;29(2):156-62.

Comparison of fractal dimension and Shannon entropy in myocytes from rats treated with histidine-tryptophan-glutamate and histidine-tryptophan cetoglutarate.

[Article in English, Portuguese]

Author information

1
Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
2
Hospital de Base São José do Rio Preto, São José do Rio Preto, SP, Brasil.
3
Santa Casa Votuporanga, Votuporanga, SP, Brasil.
4
Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brasil.

Abstract

INTRODUCTION:

Solutions that cause elective cardiac arrest are constantly evolving, but the ideal compound has not yet been found. The authors compare a new cardioplegic solution with histidine-tryptophan-glutamate (Group 2) and other one with histidine-tryptophan-cetoglutarate (Group 1) in a model of isolated rat heart.

OBJECTIVE:

To quantify the fractal dimension and Shannon entropy in rat myocytes subjected to cardioplegia solution using histidine-tryptophan with glutamate in an experimental model, considering the caspase markers, IL-8 and KI-67.

METHODS:

Twenty male Wistar rats were anesthetized and heparinized. The chest was opened, the heart was withdrawn and 40 ml/kg of cardioplegia (with histidine-tryptophan-cetoglutarate or histidine-tryptophan-glutamate solution) was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter placed in the Langendorff apparatus for 30 min with Ringer-Locke solution. Analyzes were performed for immunohistochemical caspase, IL-8 and KI-67.

RESULTS:

The fractal dimension and Shannon entropy were not different between groups histidine-tryptophan-glutamate and histidine-tryptophan-acetoglutarate.

CONCLUSION:

The amount of information measured by Shannon entropy and the distribution thereof (given by fractal dimension) of the slices treated with histidine-tryptophan-cetoglutarate and histidine-tryptophan-glutamate were not different, showing that the histidine-tryptophan-glutamate solution is as good as histidine-tryptophan-acetoglutarate to preserve myocytes in isolated rat heart.

PMID:
25140464
PMCID:
PMC4389459
DOI:
10.5935/1678-9741.20140052
[Indexed for MEDLINE]
Free PMC Article

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