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Biomed Res Int. 2014;2014:820761. doi: 10.1155/2014/820761. Epub 2014 Jul 20.

Abarema cochliacarpos extract decreases the inflammatory process and skeletal muscle injury induced by Bothrops leucurus venom.

Author information

1
Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil.
2
Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil.
3
Departamento de Fisiologia, Laboratório de Bioquímica e Química de Produtos Naturais, SE, Brazil.
4
Laboratório de Farmacologia das Toxinas, ICB, UFRJ, Universidade Federal do Rio de Janeiro, RJ, Brazil.
5
Departamento de Morfologia, Laboratório de Biologia Celular e Estrutura, Universidade Federal de Sergipe, SE, Brazil.
6
Instituto Butantan, Laboratório de Ciências Fisiológicas e Química, São Paulo, SP, Brazil.
7
Departamento de Farmácia, Laboratório de Ensaios e de Toxicidade Farmacêutica, Universidade Federal de Sergipe, SE, Brazil.
8
Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco, PE, Brazil.
9
Departamento de Biofísica e Fisiologia, Universidade Federal do Piaui, Teresina, PI, Brazil.
10
Departamento de Fisiologia, Laboratorio de Farmacologia Pré-Clinica, Universidade Federal de Sergipe, SE, Brazil ; Programa de Pós-Graduação em Biotecnologia (RENORBIO), Universidade Federal de Sergipe, SE, Brazil ; Laboratory Preclinical Pharmacology of Natural Products, Department of Physiology, Federal University of Sergipe, S/N Marechal Rondon Avenue, 49.100-000 São Cristovão, SE, Brazil.

Abstract

Snakebites are a public health problem, especially in tropical countries. However, treatment with antivenom has limited effectiveness against venoms' local effects. Here, we investigated the ability of Abarema cochliacarpos hydroethanolic extract (EAc) to protect mice against injection of Bothrops leucurus venom. Swiss mice received perimuscular venom injection and were subsequently treated orally with EAc in different doses. Treatment with EAc 100, 200, and 400 mg/kg reduced the edema induced by B. leucurus in 1%, 13%, and 39%, respectively. Although lower doses showed no antihypernociceptive effect in the Von Frey test, the higher dose significantly reduced hyperalgesia induced by the venom. Antimyotoxic activity of EAc was also observed by microscopy assessment, with treated muscles presenting preserved structures, decreased edema, and inflammatory infiltrate as compared to untreated ones. Finally, on the rotarod test, the treated mice showed better motor function, once muscle fibers were preserved and there were less edema and pain. Treated mice could stand four times more time on the rotating rod than untreated ones. Our results have shown that EAc presented relevant activities against injection of B. leucurus venom in mice, suggesting that it can be considered as an adjuvant in the treatment of envenomation.

PMID:
25136627
PMCID:
PMC4127289
DOI:
10.1155/2014/820761
[Indexed for MEDLINE]
Free PMC Article

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