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Nat Commun. 2014 Aug 19;5:4674. doi: 10.1038/ncomms5674.

Dendritic cell subsets require cis-activation for cytotoxic CD8 T-cell induction.

Author information

1
Integrated Department of Immunology, National Jewish Health and UC Denver Anschutz Campus, Denver, Colorado 80206, USA.
2
Department of Pediatrics, National Jewish Health, Denver, Colorado 80206, USA.
3
Department of Anesthesiology, UC Denver Anschutz Campus, Denver, Colorado 80206, USA.
4
Department of Medicine, National Jewish Health, Denver, Colorado 80206, USA.
5
1] Integrated Department of Immunology, National Jewish Health and UC Denver Anschutz Campus, Denver, Colorado 80206, USA [2] Department of Pediatrics, National Jewish Health, Denver, Colorado 80206, USA.

Abstract

Dendritic cells (DCs) are required for the induction of cytotoxic T cells (CTL). In most tissues, including the lung, the resident DCs fall into two types expressing the integrin markers CD103 and CD11b. The current supposition is that DC function is predetermined by lineage, designating the CD103(+) DC as the major cross-presenting DC able to induce CTL. Here we show that Poly I:C (TLR3 agonist) or R848 (TLR7 agonist) do not activate all endogenous DCs. CD11b(+) DCs can orchestrate a CTL response in vivo in the presence of a TLR7 agonist but not a TLR3 agonist, whereas CD103(+) DCs require ligation of TLR3 for this purpose. This selectivity does not extend to antigen cross-presentation for T-cell proliferation but is required for induction of cytotoxicity. Thus, we demonstrate that the ability of DCs to induce functional CTLs is specific to the nature of the pathogen-associated molecular pattern (PAMP) encountered by endogenous DC.

PMID:
25135627
PMCID:
PMC4153365
DOI:
10.1038/ncomms5674
[Indexed for MEDLINE]
Free PMC Article

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