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J Exp Bot. 2014 Nov;65(20):5867-75. doi: 10.1093/jxb/eru325. Epub 2014 Aug 18.

Microtubule array reorientation in response to hormones does not involve changes in microtubule nucleation modes at the periclinal cell surface.

Author information

1
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
2
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA vmkirik@ilstu.edu.

Abstract

Aligned microtubule arrays spatially organize cell division, trafficking, and determine the direction of cell expansion in plant cells. In response to changes in environmental and developmental signals, cells reorganize their microtubule arrays into new configurations. Here, we tested the role of microtubule nucleation during hormone-induced microtubule array reorientation. We have found that in the process of microtubule array reorientation the ratios between branching, parallel, and de-novo nucleations remained constant, suggesting that the microtubule reorientation mechanism does not involve changes in nucleation modes. In the ton2/fass mutant, which has reduced microtubule branching nucleation frequency and decreased nucleation activity of the γ-tubulin complexes, microtubule arrays were able to reorient. Presented data suggest that reorientation of microtubules into transverse arrays in response to hormones does not involve changes in microtubule nucleation at the periclinal cell surface.

KEYWORDS:

microtubule cytoskeleton; microtubule nucleation.; microtubule organization; γ-tubulin complex

PMID:
25135522
PMCID:
PMC4203123
DOI:
10.1093/jxb/eru325
[Indexed for MEDLINE]
Free PMC Article

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