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PLoS One. 2014 Aug 18;9(8):e105616. doi: 10.1371/journal.pone.0105616. eCollection 2014.

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

Author information

1
Nimni-Cordoba Tissue Engineering and Drug Discovery Laboratory, Division of Plastic and Reconstructive Surgery, Departments of Surgery and Biomedical Engineering, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.

Abstract

Development of a physiologically relevant 3D model system for cancer research and drug development is a current challenge. We have adopted a 3D culture system based on a transglutaminase-crosslinked gelatin gel (Col-Tgel) to mimic the tumor 3D microenvironment. The system has several unique advantages over other alternatives including presenting cell-matrix interaction sites from collagen-derived peptides, geometry-initiated multicellular tumor spheroids, and metabolic gradients in the tumor microenvironment. Also it provides a controllable wide spectrum of gel stiffness for mechanical signals, and technical compatibility with imaging based screening due to its transparent properties. In addition, the Col-Tgel provides a cure-in-situ delivery vehicle for tumor xenograft formation in animals enhancing tumor cell uptake rate. Overall, this distinctive 3D system could offer a platform to more accurately mimic in vivo situations to study tumor formation and progression both in vitro and in vivo.

PMID:
25133673
PMCID:
PMC4136878
DOI:
10.1371/journal.pone.0105616
[Indexed for MEDLINE]
Free PMC Article

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