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J Immunol Methods. 1989 Nov 30;124(2):205-10.

Glutaraldehyde fixation of the primary antibody-antigen complex on nitrocellulose paper increases the overall sensitivity of immunoblot assay.

Author information

1
Department of Human Genetics, University of Pennsylvania, School of Medicine, Philadelphia 19104.

Abstract

A simple method to increase the overall sensitivity of immunoblot assays is described. The method is based on fixation of the primary monoclonal antibody-antigen complex on nitrocellulose paper by a divalent crosslinker, glutaraldehyde. The fixation of the antibody-antigen complex significantly increases the sensitivity of the assay. Unexpectedly, the glutaraldehyde treatment also improves the signal/noise ratio by decreasing non-specific binding of secondary antibodies and/or tertiary reagents. Thus, a significant overall improvement of immunoblot assays is achieved by this method.

PMID:
2513357
DOI:
10.1016/0022-1759(89)90354-2
[Indexed for MEDLINE]

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