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PLoS One. 2014 Aug 18;9(8):e105204. doi: 10.1371/journal.pone.0105204. eCollection 2014.

Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells.

Author information

1
Department of Biosciences, University of Oslo, Oslo, Norway.
2
Computational Imaging and Modeling Unit, Institut Pasteur, Paris, France.
3
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institute of Health, Bethesda, Maryland, United States of America.
4
Department of Biosciences, University of Oslo, Oslo, Norway; Institute for Cancer Genetics and Informatics, Oslo University Hospital, Oslo, Norway.

Abstract

Closely related transcription factors (TFs) can bind to the same response elements (REs) with similar affinities and activate transcription. However, it is unknown whether transcription is similarly orchestrated by different TFs bound at the same RE. Here we have compared the recovery half time (t1/2), binding site occupancy and the resulting temporal changes in transcription upon binding of two closely related steroid receptors, the androgen and glucocorticoid receptors (AR and GR), to their common hormone REs (HREs). We show that there are significant differences at all of these levels between AR and GR at the MMTV HRE when activated by their ligands. These data show that two TFs bound at the same RE can have significantly different modes of action that can affect their responses to environmental cues.

PMID:
25133404
PMCID:
PMC4136857
DOI:
10.1371/journal.pone.0105204
[Indexed for MEDLINE]
Free PMC Article

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