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ACS Nano. 2014 Sep 23;8(9):8959-67. doi: 10.1021/nn501589f. Epub 2014 Aug 20.

NanoScript: a nanoparticle-based artificial transcription factor for effective gene regulation.

Author information

1
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey , Piscataway, New Jersey 08854, United States.

Abstract

Transcription factor (TF) proteins are master regulators of transcriptional activity and gene expression. TF-based gene regulation is a promising approach for many biological applications; however, several limitations hinder the full potential of TFs. Herein, we developed an artificial, nanoparticle-based transcription factor, termed NanoScript, which is designed to mimic the structure and function of TFs. NanoScript was constructed by tethering functional peptides and small molecules called synthetic transcription factors, which mimic the individual TF domains, onto gold nanoparticles. We demonstrate that NanoScript localizes within the nucleus and initiates transcription of a reporter plasmid by over 15-fold. Moreover, NanoScript can effectively transcribe targeted genes on endogenous DNA in a nonviral manner. Because NanoScript is a functional replica of TF proteins and a tunable gene-regulating platform, it has great potential for various stem cell applications.

KEYWORDS:

gene activation; nanoparticle-based genetic manipulation; nonviral delivery; synthetic transcription factors; transcription factor proteins

PMID:
25133310
PMCID:
PMC4174092
DOI:
10.1021/nn501589f
[Indexed for MEDLINE]
Free PMC Article

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