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ScientificWorldJournal. 2014;2014:584910. doi: 10.1155/2014/584910. Epub 2014 Jul 14.

Genome wide analysis of sex difference in gene expression profiles of bone formations using sfx mice and BXD RI strains.

Author information

1
Department of Orthopedic Surgery and BME, University of Tennessee Health Science Center, 956 Court Avenue, Memphis, TN 38163, USA.
2
Mudanjiang Medical College, Tongxiang Road, Aimin District, Mudanjiang City, Heilongjiang 157001, China.
3
Department of Orthopedic Surgery and BME, University of Tennessee Health Science Center, 956 Court Avenue, Memphis, TN 38163, USA ; Department of Basic Research, Inner Mongolia Medical College, Inner Mongolia 010110, China.
4
Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
5
Department of Orthopedic Surgery and BME, University of Tennessee Health Science Center, 956 Court Avenue, Memphis, TN 38163, USA ; Mudanjiang Medical College, Tongxiang Road, Aimin District, Mudanjiang City, Heilongjiang 157001, China.

Abstract

The objective of this study is to identify sex differentially expressed genes in bone using a mouse model of spontaneous fracture, sfx, which lacks the gene for L-gulonolactone oxidase (Gulo), a key enzyme in the ascorbic acid (AA) synthesis pathway. We first identified the genes that are differentially expressed in the femur between female and male in sfx mice. We then analyzed the potential gene network among those differentially expressed genes with whole genome expression profiles generated using spleens of female and male mice of a total of 67 BXD (C57BL/6J X DBA/2J) recombinant inbred (RI) and other strains. Our result indicated that there was a sex difference in the whole genome profiles in sfx mice as measured by the proportion of up- and downregulated genes. Several genes in the pathway of bone development are differentially expressed between the male and female of sfx mice. Comparison of gene network of up- and downregulated bone relevant genes also suggests a sex difference.

PMID:
25133246
PMCID:
PMC4123512
DOI:
10.1155/2014/584910
[Indexed for MEDLINE]
Free PMC Article
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