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Life Sci. 2014 Sep 26;114(1):51-6. doi: 10.1016/j.lfs.2014.07.041. Epub 2014 Aug 14.

The flavan-3-ol fraction of cocoa powder suppressed changes associated with early-stage metabolic syndrome in high-fat diet-fed rats.

Author information

1
Department of Bio-science and Engineering, Shibaura Institute of Technology Tokyo, Japan. Electronic address: nao-osa@sic.shibaura-it.ac.jp.
2
Department of Bio-science and Engineering, Shibaura Institute of Technology Tokyo, Japan. Electronic address: mf12054@sic.shibaura-it.ac.jp.
3
Department of Bio-science and Engineering, Shibaura Institute of Technology Tokyo, Japan. Electronic address: mf13033@sic.shibaura-it.ac.jp.
4
Department of Bio-science and Engineering, Shibaura Institute of Technology Tokyo, Japan. Electronic address: shibatam@sic.shibaura-it.ac.jp.

Abstract

AIMS:

Previous epidemiological studies have suggested that ingestion of chocolate reduces the risk of cardiovascular disease. In the present study, we examined the effects of flavan-3-ols derived from cocoa on blood pressure, lipolysis, and thermogenesis in rats fed a high-fat diet and that showed early signs of metabolic syndrome.

MAIN METHODS:

The rats were divided into three groups, and fed either normal diet (normal), 60% fat high-fat diet (HFD), or HFD containing 0.2% flavan-3-ols (HFD-flavan) for 4 weeks. At the end of the feeding period, blood pressure was measured and animals were sacrificed under anesthesia. Lipolysis and thermogenesis-related protein levels were measured in several tissues by Western blotting, and mitochondrial DNA copy number was measured by RT-PCR.

KEY FINDINGS:

Mean blood pressure and epididymal adipose tissue weight of HFD-flavan were significantly lower compared with those of HFD. Uncoupling protein (UCP)1 in brown adipose tissue and UCP3 in gastrocnemius of HFD-flavan were significantly increased compared with those of HFD group. Carnitine palmitoyltransferase (CPT) 2 levels in liver and medium-chain acyl-CoA dehydrogenase (MCAD) levels in gastrocnemius and liver were significantly increased by the supplementation of flavan-3-ols.

SIGNIFICANCE:

In addition to having hypotensive effects, flavan-3-ols enhance thermogenesis and lipolysis and consequently reduce white adipose tissue weight gain in response to high-fat diet feeding.

KEYWORDS:

Flavan-3-ols; Lipolysis; Thermogenesis; Uncoupling proteins

PMID:
25132363
DOI:
10.1016/j.lfs.2014.07.041
[Indexed for MEDLINE]

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