Format

Send to

Choose Destination
Transplant Proc. 2014 Jul-Aug;46(6):2125-32. doi: 10.1016/j.transproceed.2014.06.039.

Evaluation of epithelial chimerism after bone marrow mesenchymal stromal cell infusion in intestinal transplant patients.

Author information

1
Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey. Electronic address: selcukkilinc79@gmail.com.
2
Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, Ohio, USA.
3
Bio-acoustic MEMS in Medicine Laboratory, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, USA.
4
Institute of Forensic Medicine, Izmir, Turkey.
5
Department of General Surgery, Dr Lütfi Kırdar Kartal Training and Research Hospital, Istanbul, Turkey.
6
Organ Transplantation Center, Department of General Surgery, Tepecik Training and Research Hospital, Izmir, Turkey.
7
Department of Cardiology, Tepecik Training and Research Hospital, Izmir, Turkey.
8
Department of Pathology, Tepecik Training and Research Hospital, Izmir, Turkey.
9
Department of Pediatrics, Tepecik Training and Research Hospital, Izmir, Turkey.
10
Department of Biochemistry, Tepecik Training and Research Hospital, Izmir, Turkey.
11
Bio-acoustic MEMS in Medicine Laboratory, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, USA; Harvard-Massachusetts Institute of Technology (MIT) Health Sciences and Technology, Cambridge, Massachusetts, USA. Electronic address: udemirci@rics.bwh.harvard.edu.

Abstract

Intestinal transplantation is the most effective treatment for patients with short bowel syndrome and small bowel insufficiencies. We evaluated epithelial chimerism after infusion of autologous bone marrow mesenchymal stromal cells (BMSCs) in patients undergoing cadaveric donor isolated intestinal transplantation (I-ITx). BMSCs were isolated from patients' bone marrow via iliac puncture and expanded in vitro prior to infusion. Two out of the 3 patients were infused with autologous BMSCs, and small intestine tissue biopsies collected post-operatively were analyzed for epithelial chimerism using XY fluorescent in situ hybridization and short tandem repeat polymerase chain reaction. We observed epithelial chimeric effect in conditions both with and without BMSC infusion. Although our results suggest a higher epithelial chimerism effect with autologous BMSC infusion in I-ITx, the measurements in multiple biopsies at different time points that demonstrate the reproducibility of this finding and its stability or changes in the level over time would be beneficial. These approaches may have potential implications for improved graft survival, lower immunosuppressant doses, superior engraftment of the transplanted tissue, and higher success rates in I-ITx.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center