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Carbohydr Polym. 2014 Nov 4;112:556-62. doi: 10.1016/j.carbpol.2014.06.035. Epub 2014 Jun 20.

Dextran synthesized by Leuconostoc mesenteroides BD1710 in tomato juice supplemented with sucrose.

Author information

1
State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China.
2
Shanghai Engineering Research Center of Dairy Biotechnology, China.
3
Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China.
4
State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China. Electronic address: wuzhengjun@brightdairy.com.

Abstract

The characteristics of the growth of Leuconostoc mesenteroides BD1710 and the synthesis of dextran in tomato juice supplemented with 15% sucrose were assayed. L. mesenteroides BD1710 could synthesize approximately 32 g L(-1) dextran in the tomato-juice-sucrose medium when cultured at 28 °C for 48 h, which was on the same level as the dextran yield in a chemically defined medium. The viscosity of the cultured tomato-juice-sucrose medium with various dextran contents was also measured. The results of the monosaccharide composition, molecular-weight distribution, Fourier transform infrared spectra (FTIR) and nuclear magnetic resonance spectra (NMR) showed that the polysaccharide synthesized by L. mesenteroides BD1710 in the tomato-juice-sucrose medium was dextran with a peak molecular weight of 6.35 × 10(5)Da, a linear backbone composed of consecutive α-(1 → 6)-linked d-glucopyranosyl units and approximately 6% α-(1 → 3) branches.

KEYWORDS:

Dextran; Leuconostoc mesenteroides BD1710; Tomato juice sucrose medium

PMID:
25129781
DOI:
10.1016/j.carbpol.2014.06.035
[Indexed for MEDLINE]

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