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Curr Opin Biotechnol. 2015 Feb;31:1-9. doi: 10.1016/j.copbio.2014.07.008. Epub 2014 Aug 15.

High throughput screening of enzyme activity with mass spectrometry imaging.

Author information

1
Dept. of Chemistry, University of California, Berkeley, CA 94720, USA.
2
Lawrence Berkeley National Lab, One Cyclotron Road, Berkeley, CA 94720, USA.
3
Lawrence Berkeley National Lab, One Cyclotron Road, Berkeley, CA 94720, USA; Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA. Electronic address: trnorthen@lbl.gov.

Abstract

Mass spectrometry imaging (MSI) has found a diversity of applications ranging from localizing metabolites and proteins in tissues to investigating microbial interactions, and as a result is perhaps the fastest growing subfield of mass spectrometry. Advances in surface mass spectrometry technologies are equally applicable to the analysis of arrayed samples. One promising field in which this capacity has been leveraged is the high-throughput analysis of enzyme activity, an important step in the development of a wide range of biotechnologies. This review article describes several emerging approaches that seek to improve the quality and scope of this application of MSI.

PMID:
25129648
DOI:
10.1016/j.copbio.2014.07.008
[Indexed for MEDLINE]

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