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Curr Opin Chem Biol. 2014 Aug;21:144-53. doi: 10.1016/j.cbpa.2014.07.015. Epub 2014 Aug 15.

Turning the spotlight on protein-lipid interactions in cells.

Author information

1
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, United States.
2
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, United States. Electronic address: hhang@rockefeller.edu.

Abstract

Protein function is largely dependent on coordinated and dynamic interactions of the protein with biomolecules including other proteins, nucleic acids and lipids. Although powerful methods for global profiling of protein-protein and protein-nucleic acid interactions are available, proteome-wide mapping of protein-lipid interactions is still challenging and rarely performed. The emergence of bifunctional lipid probes with photoactivatable and clickable groups offers new chemical tools for globally profiling protein-lipid interactions under cellular contexts. In this review, we summarize recent advances in the development of bifunctional lipid probes for studying protein-lipid interactions. We also highlight how in vivo photocrosslinking reactions contribute to the characterization of lipid-binding proteins and lipidation-mediated protein-protein interactions.

PMID:
25129056
PMCID:
PMC4206213
DOI:
10.1016/j.cbpa.2014.07.015
[Indexed for MEDLINE]
Free PMC Article

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