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Nat Methods. 2014 Oct;11(10):1037-40. doi: 10.1038/nmeth.3068. Epub 2014 Aug 17.

Multiplexed aberration measurement for deep tissue imaging in vivo.

Author information

1
1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2] State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, China. [3].
2
1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2].
3
1] Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA. [2].
4
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.

Abstract

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

PMID:
25128976
PMCID:
PMC4180771
DOI:
10.1038/nmeth.3068
[Indexed for MEDLINE]
Free PMC Article

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