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Clin Immunol. 2014 Oct;154(2):164-77. doi: 10.1016/j.clim.2014.08.003. Epub 2014 Aug 14.

Influenza virus-like particle vaccines made in Nicotiana benthamiana elicit durable, poly-functional and cross-reactive T cell responses to influenza HA antigens.

Author information

1
Medicago inc., 1020 Route de l'Église, Bureau 600, Québec, Qc G1V 3V9, Canada. Electronic address: landryn@medicago.com.
2
Medicago inc., 1020 Route de l'Église, Bureau 600, Québec, Qc G1V 3V9, Canada; Research Institute of the McGill University Health Center, Montreal General Hospital, 1650 Cedar Avenue, Montreal, Qc H3G 1A4, Canada. Electronic address: pillets@medicago.com.
3
ImmuneCarta, 201 President-Kennedy, Suite PK-3900, Montreal, Qc H2X 3Y7, Canada. Electronic address: dfavre@gmail.com.
4
ImmuneCarta, 201 President-Kennedy, Suite PK-3900, Montreal, Qc H2X 3Y7, Canada. Electronic address: jpoulin@immunecarta.com.
5
Medicago inc., 1020 Route de l'Église, Bureau 600, Québec, Qc G1V 3V9, Canada. Electronic address: trepaniers@medicago.com.
6
ImmuneCarta, 201 President-Kennedy, Suite PK-3900, Montreal, Qc H2X 3Y7, Canada. Electronic address: byassine@immunecarta.com.
7
Research Institute of the McGill University Health Center, Montreal General Hospital, 1650 Cedar Avenue, Montreal, Qc H3G 1A4, Canada. Electronic address: brian.ward@mcgill.ca.

Abstract

Cell-mediated immunity plays a major role in long-lived, cross-reactive protection against influenza virus. We measured long-term poly-functional and cross-reactive T cell responses to influenza hemagglutinin (HA) elicited by a new plant-made Virus-Like Particle (VLP) vaccine targeting either H1N1 A/California/7/09 (H1) or H5N1 A/Indonesia/5/05 (H5). In two independent clinical trials, we characterized the CD4(+) and CD8(+) T cell homotypic and heterotypic responses 6 months after different vaccination regimens. Responses of VLP-vaccinated subjects were compared with placebo and/or a commercial trivalent inactivated vaccine (TIV:Fluzone™) recipients. Both H1 and H5 VLP vaccines elicited significantly greater poly-functional CD4(+) T cell responses than placebo and TIV. Poly-functional CD8(+) T cell responses were also observed after H1 VLP vaccination. Our results show that plant-made HA VLP vaccines elicit both strong antibody responses and poly-functional, cross-reactive memory T cells that persist for at least 6 months after vaccination.

KEYWORDS:

Cell-mediated immunity; Influenza; Long-term memory response; Nicotiana benthamiana; Vaccine; Virus-like particle

PMID:
25128897
DOI:
10.1016/j.clim.2014.08.003
[Indexed for MEDLINE]
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