Chemical composition, anti-inflammatory activity and cytotoxic activity of the liposoluble constituents from different parts of Acanthopanax evodiaefolius by the Herbal Blitzkrieg Extractor

Environ Toxicol Pharmacol. 2014 Sep;38(2):406-11. doi: 10.1016/j.etap.2014.07.005. Epub 2014 Jul 16.

Abstract

The liposoluble constituents of leaves (LCL) and stem barks (LCSB) from Acanthopanax evodiaefolius Franch were extracted by Herbal Blitzkrieg Extractor (HBE), and their chemical composition was analyzed by GC-MS. 18 compounds were identified, representing 71.98% of LCL, while 35 compounds were identified, accounting for 98.28% of the LCSB. Their cytotoxic activity and inhibitory effect on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells were tested and the LCL showed significant NO, IL-6 and TNF-α inhibition activity. These results provide the scientific basis for looking for new natural anti-inflammatory substances and exploiting and developing resources of A. evodiaefolius Franch.

Keywords: Acanthopanax evodiaefolius; Anti-inflammatory; Cytotoxic activity; Herbal Blitzkrieg Extractor; Liposoluble constituents; Nitric oxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line
  • Cell Survival / drug effects
  • Eleutherococcus / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Mice
  • Nitric Oxide / metabolism
  • Plant Bark / chemistry
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Plant Stems / chemistry
  • Plants, Medicinal / chemistry
  • Technology, Pharmaceutical / methods

Substances

  • Anti-Inflammatory Agents
  • Lipopolysaccharides
  • Plant Extracts
  • Nitric Oxide