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Tissue Eng Part C Methods. 2015 Mar;21(3):292-302. doi: 10.1089/ten.TEC.2014.0296. Epub 2014 Sep 19.

Application and assessment of optical clearing methods for imaging of tissue-engineered neural stem cell spheres.

Author information

1
1 Center for Biomedical Engineering, Brown University , Providence, Rhode Island.

Abstract

Three-dimensional (3D) cell culture is an important tool that facilitates biological discoveries by bridging the divide between standard two-dimensional cell culture and the complex, high-cell-density in vivo environment. Typically, the internal structures of 3D tissue-engineered samples are visualized through an involved process of physical sectioning, immunostaining, imaging, and computational reconstruction. However, recent progress in tissue-clearing methods has improved optical-imaging-depth capabilities in whole embryos and brains by reducing tissue opacity and light scattering, thus decreasing the need for physical sectioning. In this study, we assessed the application of the recently published clearing techniques Clear(T2), Scale, and SeeDB to tissue-engineered neural spheres. We found that scaffold-free self-assembled adult hippocampal neural stem cell spheres of 100-μm diameter could be optically cleared and imaged using either Clear(T2) or Scale, while SeeDB only marginally improved imaging depth. The Clear(T2) protocol maintained sphere size, while Scale led to sample expansion, and SeeDB led to sample shrinkage. Additionally, using Clear(T2) we cleared and successfully imaged spheres of C6 glioma cells and spheres of primary cortical neurons. We conclude that Clear(T2) is the most effective protocol of those tested at clearing neural spheres of various cell types and could be applied to better understand neural cell interactions in 3D tissue-engineered samples.

PMID:
25128373
PMCID:
PMC4346659
DOI:
10.1089/ten.TEC.2014.0296
[Indexed for MEDLINE]
Free PMC Article

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