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PLoS One. 2014 Aug 15;9(8):e105191. doi: 10.1371/journal.pone.0105191. eCollection 2014.

Generation and behavior characterization of CaMKIIβ knockout mice.

Author information

1
Sanders-Brown Center on Aging, University of Kentucky, Lexington, Kentucky, United States of America.
2
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.
3
Laboratoire d'Innovation Thérapeutique, University of Strasbourg, Strasbourg, France.
4
Sanders-Brown Center on Aging, University of Kentucky, Lexington, Kentucky, United States of America; Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky, United States of America.

Abstract

The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO) mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.

PMID:
25127391
PMCID:
PMC4134274
DOI:
10.1371/journal.pone.0105191
[Indexed for MEDLINE]
Free PMC Article

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