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Biochim Biophys Acta. 2014 Nov;1837(11):1861-1869. doi: 10.1016/j.bbabio.2014.08.001. Epub 2014 Aug 12.

Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila.

Author information

1
BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland; Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
2
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
3
BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland.
4
BioMediTech and Tampere University Hospital, University of Tampere, FI-33014, Finland; Research Program of Molecular Neurology, University of Helsinki, FI-00014, Finland. Electronic address: howard.t.jacobs@uta.fi.

Abstract

An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits.

KEYWORDS:

Ageing; Alternative respiratory chain enzymes; Gene therapy; Oxidative stress; Tunicate

PMID:
25124484
DOI:
10.1016/j.bbabio.2014.08.001
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