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J Neurosci. 2014 Aug 13;34(33):10884-91. doi: 10.1523/JNEUROSCI.4795-13.2014.

High-NaCl perception in Drosophila melanogaster.

Author information

1
Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France georges.alves@u-bourgogne.fr.
2
Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Université de Bourgogne, 21000 Dijon, France.

Abstract

Salt is a fundamental nutrient that is required for many physiological processes, including electrolyte homeostasis and neuronal activity. In mammals and Drosophila, the detection of NaCl induces two different behaviors: low-salt concentrations provide an attractive stimulus, whereas high-salt concentrations are avoided. We identified the gene called serrano (sano) as being expressed in the sensory organs of Drosophila larvae. A transgenic reporter line showed that sano was coexpressed with Gr66a in a subset of gustatory neurons in the terminal organ of third-instar larvae. The disruption of sano gene expression in gustatory neurons led to the specific loss of high-salt concentration avoidance in larvae, whereas the detection of other attractive or aversive substances was unaffected. Moreover, using a cellular marker sensitive to calcium levels, Sano function was shown to be required for neuronal activity in response to high-salt concentrations. In these neurons, the loss of the DEG/ENaC channel PPK19 function also eliminated the cellular response to high-salt concentrations. Our study revealed that PPK19 and Sano are required in the neurons of the larval gustatory organs for the detection of high-salt concentrations.

KEYWORDS:

Drosophila melanogaster; behavior; chemosensory system; larva; salt; taste

PMID:
25122890
DOI:
10.1523/JNEUROSCI.4795-13.2014
[Indexed for MEDLINE]
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