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PLoS One. 2014 Aug 14;9(8):e99519. doi: 10.1371/journal.pone.0099519. eCollection 2014.

Metabolome analysis of Drosophila melanogaster during embryogenesis.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
2
Department of Applied Biology, Insect Biomedical Research Center, Kyoto Institute of Technology, Sakyo-ku, Kyoto, Japan.

Abstract

The Drosophila melanogaster embryo has been widely utilized as a model for genetics and developmental biology due to its small size, short generation time, and large brood size. Information on embryonic metabolism during developmental progression is important for further understanding the mechanisms of Drosophila embryogenesis. Therefore, the aim of this study is to assess the changes in embryos' metabolome that occur at different stages of the Drosophila embryonic development. Time course samples of Drosophila embryos were subjected to GC/MS-based metabolome analysis for profiling of low molecular weight hydrophilic metabolites, including sugars, amino acids, and organic acids. The results showed that the metabolic profiles of Drosophila embryo varied during the course of development and there was a strong correlation between the metabolome and different embryonic stages. Using the metabolome information, we were able to establish a prediction model for developmental stages of embryos starting from their high-resolution quantitative metabolite composition. Among the important metabolites revealed from our model, we suggest that different amino acids appear to play distinct roles in different developmental stages and an appropriate balance in trehalose-glucose ratio is crucial to supply the carbohydrate source for the development of Drosophila embryo.

PMID:
25121768
PMCID:
PMC4133167
DOI:
10.1371/journal.pone.0099519
[Indexed for MEDLINE]
Free PMC Article

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