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Methods Mol Biol. 2014;1200:491-9. doi: 10.1007/978-1-4939-1292-6_40.

A new structure determination method of lectins using a selenium-containing sugar ligand.

Author information

1
Photon Factory, Structural Biology Research Center, IMSS, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki, 305-0801, Japan.

Abstract

Phase determination is essential for solving X-ray crystal structures of proteins and their complexes. Conventional phase-determination methods using heavy atoms (Pt, Au, Hg, etc.) or the selenium (Se) atom are routinely utilized in structure determination of protein crystals. Here, we describe an alternative phase-determination method for proteins such as lectins in which a Se-containing glycan is used as a ligand. In this technique, the Se atoms are simply introduced into the protein crystal as a complex, and the phase of the protein can be determined using anomalous signals from the Se-containing sugar.

PMID:
25117259
DOI:
10.1007/978-1-4939-1292-6_40
[Indexed for MEDLINE]

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