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Sci Rep. 2014 Aug 13;4:6043. doi: 10.1038/srep06043.

The newt reprograms mature RPE cells into a unique multipotent state for retinal regeneration.

Author information

1
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8572 Japan.
2
Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8572 Japan.
3
Graduate School of Engineering, Utsunomiya University, Yoto 7-1-2, Utsunomiya, Tochigi 321-8585, Japan.

Abstract

The reprogramming of retinal pigment epithelium (RPE) cells in the adult newt immediately after retinal injury is an area of active research for the study of retinal disorders and regeneration. We demonstrate here that unlike embryonic/larval retinal regeneration, adult newt RPE cells are not directly reprogrammed into retinal stem/progenitor cells; instead, they are programmed into a unique state of multipotency that is similar to the early optic vesicle (embryo) but preserves certain adult characteristics. These cells then differentiate into two populations from which the prospective-neural retina and -RPE layers are formed with the correct polarity. Furthermore, our findings provide insight into the similarity between these unique multipotent cells in newts and those implicated in retinal disorders, such as proliferative vitreoretinopathy, in humans. These findings provide a foundation for biomedical approaches that aim to induce retinal self-regeneration for the treatment of RPE-mediated retinal disorders.

PMID:
25116407
PMCID:
PMC4131214
DOI:
10.1038/srep06043
[Indexed for MEDLINE]
Free PMC Article

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