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Front Biol (Beijing). 2013 Apr 1;8(2):216-227.

Current technologies to identify protein kinase substrates in high throughput.

Author information

1
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
2
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA ; Department of Medicinal Chemistry & Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA ; Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA ; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.

KEYWORDS:

high throughput screening; in vitro kinase assay; kinase substrate; mass spectrometry; phosphoproteomics; phosphorylation

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