Format

Send to

Choose Destination
See comment in PubMed Commons below
Biotechniques. 2014 Aug 1;57(2):81-7. doi: 10.2144/000114198. eCollection 2014 Aug.

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification.

Author information

1
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia; All-Russia Institute of Agricultural Biotechnology, Moscow, Russia.
2
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Evrogen JSC, Moscow, Russia.
3
All-Russia Institute of Agricultural Biotechnology, Moscow, Russia; Syntol JSC, Moscow, Russia.
4
The Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.

Abstract

The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used. We have compared the performance of conventional Taq and Bst DNA polymerases to a novel Taq DNA polymerase mutant (SD DNA polymerase), which has a strong strand displacement activity, in PCR (including amplification of GC-rich and complex secondary structure templates), long-range PCR (LR PCR), loop-mediated amplification (LAMP), and polymerase chain displacement reaction (PCDR). Our results demonstrate that the strand displacement activity of SD DNA polymerase, in combination with the robust polymerase activity, provides a notable improvement in the sensitivity and efficiency of all these methods.

KEYWORDS:

DNA polymerase; LAMP; PCDR; PCR; isothermal amplification; quantitative amplification; real-time amplification; strand displacement

PMID:
25109293
DOI:
10.2144/000114198
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Informa Healthcare, USA
    Loading ...
    Support Center