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Nat Methods. 2014 Sep;11(9):923-6. doi: 10.1038/nmeth.3067. Epub 2014 Aug 10.

Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser.

Author information

1
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
2
Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
3
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
4
Department of Chemistry - Ångström Laboratory, Uppsala University, Uppsala, Sweden.
5
1] Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. [2] Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
6
1] Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany. [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Hamburg, Germany.
7
Department of Physics, Arizona State University, Tempe, Arizona, USA.
8
1] Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Hamburg, Germany. [2] Max-Planck-Institut für Kernphysik, Heidelberg, Germany.
9
PULSE Institute for Ultrafast Energy Science, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
10
Department of Physics, Technical University of Denmark, Lyngby, Denmark.
11
1] Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany. [2] Department of Physics, Arizona State University, Tempe, Arizona, USA.
12
1] Department of Physics, Technical University of Denmark, Lyngby, Denmark. [2] Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark.
13
Lawrence Livermore National Laboratory, Livermore, California, USA.
14
Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
15
BioCARS, University of Chicago, Chicago, Illinois, USA.
16
1] Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland. [2] Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland.
17
1] Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany. [2] Department of Physics, University of Hamburg, Hamburg, Germany. [3] Centre for Ultrafast Imaging, Hamburg, Germany.

Abstract

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.

PMID:
25108686
PMCID:
PMC4149589
DOI:
10.1038/nmeth.3067
[Indexed for MEDLINE]
Free PMC Article

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