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Trends Genet. 2014 Sep;30(9):418-26. doi: 10.1016/j.tig.2014.07.001. Epub 2014 Aug 6.

Ten years of next-generation sequencing technology.

Author information

1
Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France. Electronic address: vandijk@cgm.cnrs-gif.fr.
2
Centre de Génétique Moléculaire - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.
3
Plateforme Intégrée IMAGIF - CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France.

Abstract

Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized NGS and paved the way for the development of a large number of novel NGS applications in basic science as well as in translational research areas such as clinical diagnostics, agrigenomics, and forensic science. Here we provide an overview of the evolution of NGS and discuss the most significant improvements in sequencing technologies and library preparation protocols. We also explore the current landscape of NGS applications and provide a perspective for future developments.

KEYWORDS:

ChIP-seq; DNA-seq; NGS library preparation; Next-generation sequencing (NGS); RNA-seq; genomics

PMID:
25108476
DOI:
10.1016/j.tig.2014.07.001
[Indexed for MEDLINE]

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